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Journal of Pharmacognosy and Phytochemistry
ethylhe xyl)ester co mpound were present in the metha nolic
extract of Phyllanthus acidus
[24]
. Several fatty acid s and
phenolic co mpounds were kno wn in GC - M S analysis of
methanol extract which ma y be responsible for the
antimicrobial activity
[25 ]
. T he previous reports sho wed that
1,2 - Benzenedicarboxylic acid, butyl 2 - co mpounds obtained
fro m plant extract sho wed various biological activities like
antibacterial, antifungal, antiviral and anticancer
[19]
and also
reported metha nolic extract of P. amarus was screened for the
presence of se veral bioactive c ompo unds
[26]
.
4. Conclusions
In the present stud y, phyto che mical co mpone nts suc h as
steroids/sterols, cardiac glycosides, saponins, alkaloids,
tannins, phe nols, flavonoids and terpenoids were present in
the metha nolic leaf e xtract of Phyllanthus debilis . FTIR
spectrum a nalysis of a me tha nolic leaf e xtract of Phyllanthus
debilis indicated the presenc e of different phytofunc tional
groups suc h as a lka nes, ethe rs, a mines, alde hydes, a lipha tic
bromo and alipha tic chlor o compounds and aro matic
compounds. GC - M S chro matogra m analysis of the
me thanolic lea f extract of Phyllanthus debilis revealed the 9
major bioactive co mpounds, which ha ve a ntio xidant,
antimicrobial and antifunga l activity. Usually medicinal
plants contain numerous sec ondary metabolites, which are
plays a pivotal role to control the gro wth of the
microorganisms. T herefore, based on the results it ca n be
concluded that the metha nolic extract of Phyllanthus debilis
ma y hold enor mo us resourc e of phar mace utical properties
which might be used for natur al therap y.
Table 2: Biochemical Compounds detected in methanol leaf extr act of Phyllanthus debilis by GC - MS analysis, and its Biological activit y
S.
No
R.T Name of the compounds M . F M .W
Category of
compound
Biologocal activity
1. 14.62 Phenol, 2,4 - bis(1,1) - dimeth yleth yl C
14
H
22
O 602.26
Phenolic
compound
Antibacterial, antifugal, antimalarial and
antioxidant activity
2. 18.03 Flavone C
15
H
10
O
2
222.24
Flavonoid
compound
Anticancer, antioxidant, anti - inflammator y,
Antibacterial, Anti viral, Hepatoprotetive activit y
3. 20.22 Tridecanoic acid, Methyl ester C
14
H
28
O
2
228.37 Fatty acid Cancer preventive
4. 21.47 13 - Hexyloxacyclotridec - 10 - en - 2 - one C
18
H
32
O
2
280.45 Fatty acid Anti microbial activity
5. 21.8 Phytol C
20
H
40
O 296.53 Diterpene
Anticancer, antioxidant, anti - inflammator y,
diuretic, antitumor, Chem Opreventive ,
antimicrobial, use in vaccine formulations
6 22.67 Oleic acid C
18
H
34
O
2
282 Fatty acid Antibacterial
7. 23.73
2H - Naphthalen - 1 - one, 3,4 - dihydro - 6 - methoxy -
2 - (4 - methoxybenzylidene) -
C
19
H
18
O
3
294.12 Keto compounds Antiaggregant and antifungal
8. 25.15 Benzonaphth - 1 - one, 6 - acetate - 9 - phenyl C2
1
H
14
O
3
314.34 Keto compounds Anticancer
9. 26.32
1,2,Benzenedicarboxylic acid, Mono(2 -
ethylhexyl)ester
C
16
H
22
O
4
278.34
P lastizer
compound
Anti microbial activity
R. T: Retention Ti me, M.F: Molecular Formula, M.W: Molecular Weight, ∗Source: Dr. Duke's Phytochemical and Ethno botanical Databases
(online database), https://pubchem.ncbi.nlm.nih.gov
5. Conflict of Interests
The authors declare that there is no conflict of interests
regarding the publication of this paper.
6. Acknowledgments
The authors are thankful to the Tamil Nadu State Council for
Science and Technology (TNSCST), Chennai (reference no:
TNSCST/RFRS/VR/02/2015-2016) for providing financial
assistance and the Department of Botany, Jamal Mohamed
College, Tiruchirappalli, Tamil Nadu, India, for the necessary
facilities.
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